Preferential isolation of fragmented DNA enhances the detection of circulating mutated k-ras DNA.

al. Comparison of two strategies for administering nevirapine to prevent perinatal HIV transmission in high-prevalence, resource-poor settings. Simultaneous quantitative assay of six HIV protease inhibitors, one metab-olite, and two non-nucleoside reverse transcriptase inhibitors in human plasma by isocratic reversed-phase liquid chromatography. High-performance liquid chromatographic assay to determine the plasma levels of HIV-protease inhibitors (amprenavir, indinavir, nelfinavir, ritonavir and saquina-vir) and the non-nucleoside reverse transcriptase inhibitor (nevirapine) after liquid-liquid extraction. Simple and rapid determination of nevirapine in human serum by reversed-phase high-performance liquid chromatography. monitoring of antiretroviral therapy for HIV-1 infection: method validation and results of a pilot study. Hekster YA. Simultaneous determination of the HIV-protease inhibitors indina-vir, nelfinavir, saquinavir and ritonavir in human plasma by reversed-phase high-performance liquid chromatography. et al. The steady-state pharmacokinetics of nevirapine during once daily and twice daily dosing in HIV-1-infected individuals. et al. Timing of the maternal drug dose and risk of perinatal HIV transmission in the setting of intrapartum and neonatal singe-dose nevirapine. multicenter randomized controlled trial of nevirapine versus a combination of zidovudine and lamivudine to reduce intrapartum and early postpartum mother-to-child transmission of human immunodeficiency virus type 1. Intrapartum and neonatal single-dose nevirapine compared with zidovudine for prevention of mother-to-child transmission of HIV-1 in Kampala, Uganda: HIVNET 012 randomized trial. Efficacy of antenatal zidovudine in reducing perinatal transmission of human immunodeficiency virus type 1. Reduction of maternal-infant transmission of human immunodeficiency virus type 1 with zidovudine treatment. Cost-effective use of nevirapine to prevent vertical HIV transmission in sub-Saharan Africa. effectiveness of single-dose nevirapine regimen for mother and infant to decrease vertical HIV-1 transmission in sub-Saharan Africa. In the course of studying the incidence of k-ras mutations in DNA in the circulation of patients with colorectal disease, we observed that our ability to detect mutated k-ras DNA in serum varied with the method of DNA isolation. Two methods of DNA isolation were used in this study. One was the QIAamp DNA Blood Midi Kit (Qiagen, Inc.), which has been widely used for isolating circulating DNA from serum or plasma (1–5). The other is the modified Guanidine/Promega Resin (G/R) method that was developed to isolate DNA from urine (6). To compare the applicability of the Qiagen and G/R methods for isolation of circulating DNA, we purified DNA from aliquots of serum from six patients with known mutant k-ras-positive colorectal disease (colorectal cancer or adenomatous polyps) and analyzed it for the presence of mutated k-ras …